Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (2024)

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Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (1)

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  • Primary Antibodies
  • Ly-6G Antibodies

Invitrogen

15 Published Figures
20 References

View all (38) Ly-6G antibodies

Datasheet

Protocols

Tech Support

Cite Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780, eBioscience™

Datasheet

Protocols

Tech Support

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (2)

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Ly-6G Antibody (47-9668-82) in Flow

C57BL/6 mouse bone marrow cells were stained with Ly-6C Monoclonal Antibody, eFluor 450 (Product # 48-5932-82) and 0.125 µg of Rat IgG2a kappa Isotype Control, APC-eFluor 780 (Product # 47-4321-82) (left) or 0.125 µg of Ly-6G Monoclonal Antibody, APC-eFluor 780 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more

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Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (4)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (5)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (6)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (7)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (8)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (9)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (10)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (11)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (12)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (13)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (14)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (15)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (16)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (17)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (18)

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (19)

Product Details

47-9668-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 2 publications2 publications

Immunohistochemistry (Paraffin) (IHC (P))

-

Immunocytochemistry(ICC/IF)

-

View 1 publication1 publication

Flow Cytometry(Flow)

0.25 µg/test

View 16 publications16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat/ IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

1A8-Ly6g

Conjugate

APC-eFluor™ 780

  • Alexa Fluor 700
  • APC
  • Biotin
  • Brilliant UV 395
  • Brilliant UV 737
  • Brilliant Violet 421
  • Brilliant Violet 480
  • Brilliant Violet 786
  • eFluor 450
  • FITC
  • Functional Grade
  • PE
  • PE-Cyanine5
  • PE-Cyanine7
  • PE-eFluor 610
  • PerCP-eFluor 710
  • Super Bright 436
  • Super Bright 600
  • Super Bright 702

Excitation/Emission Max

756/785 nmView spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Wet ice

RRID

AB_2802291

Product Specific Information

Description: The monoclonal antibody 1A8-Ly6g reacts with mouse Ly-6G, which is also known as Gr-1. Ly-6G is a 25-kDa GPI protein expressed exclusively by neutrophils. Studies suggest an important role in neutrophil infiltration, recruitment and migration. Ly-6G associates with B2 integrins CD11a and CD11b and can attenuate B2 integrin expression and function. The ligand for Ly-6G is currently unknown.

Applications Reported: This 1A8-Ly6g antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1A8-Ly6g antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Target Information

Ly6G (Lymphocyte antigen 6 complex locus G6D) is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. The Ly6G protein has a molecular weight of approximately 21-25 kDa and is expressed by myeloid derived cells in the bone marrow. Ly6G is a good marker for detection of peripheral neutrophils, monocytes and granulocytes. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. The physiological role of Ly6G remains unclear but studies have shown its involvement in anti-tumor response, and studies with neutrophils suggest that Ly6G ligation may have an inhibitory effect on local immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

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Bioinformatics

Protein Aliases:Ly-6G; Ly-6G.1; Lymphocyte antigen 6G

View moreView less

Gene Aliases:Gr-1; Gr1; Ly-6G; Ly6g

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It has to be done as per old AB suggested Products section.

Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (21)

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Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (22)

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Ly-6G Monoclonal Antibody (1A8-Ly6g), APC-eFluor™ 780 (47-9668-82) (2024)

FAQs

What disease is diagnosed with monoclonal antibodies? ›

In the simplest test monoclonal antibodies to a known pathogen are added to a sample of an unknown pathogen. If the antibodies attach to antigens and make cells clump together, then the unknown pathogen is identified as the monoclonal antibody pathogen.

What do monoclonal antibodies indicate? ›

Monoclonal antibodies are clones of your body's antibodies that are made in a laboratory, meant to stimulate your immune system. Monoclonal antibodies as therapies are more targeted than some other types of treatments and have been more successful at treating some types of diseases, including some cancers.

What is Ly6G a marker for? ›

Ly6G Antibody - A Marker for Monocytes, Granulocytes and Neutrophils.

What is a monoclonal antibody marker? ›

Listen to pronunciation. (MAH-noh-KLOH-nul AN-tee-BAH-dee) A type of protein that is made in the laboratory and can bind to certain targets in the body, such as antigens on the surface of cancer cells.

What autoimmune disease does monoclonal antibodies treat? ›

Monoclonal antibodies are a class of drugs used to treat inflammatory autoimmune disorders (conditions in which the immune system attacks the healthy parts of the body and causes pain, swelling, and damage), skin disorders, certain types of arthritis (such as rheumatoid arthritis, psoriatic arthritis, juvenile ...

What are the potential risks of monoclonal antibodies? ›

Some mAbs can have side effects that are related to the antigens they target. For example: Bevacizumab (Avastin) is an mAb that targets a protein called VEGF that affects tumor blood vessel growth. It can cause side effects such as high blood pressure, bleeding, poor wound healing, blood clots, and kidney damage.

What can monoclonal antibodies help detect? ›

Flagging cancer cells.

Some immune system cells depend on antibodies to locate the target of an attack. Cancer cells that are coated in monoclonal antibodies may be more easily detected and targeted for destruction.

What are the three types of monoclonal antibodies? ›

We have characterized three types of monoclonal antibodies, namely: (1) antibodies that bind to NGF and inhibit its binding to target cells and its biological activity in culture (type A); (2) antibodies that bind to and precipitate NGF but do not inhibit its binding to target cells or its biological activity (type B); ...

When should you get monoclonal antibodies? ›

Monoclonal antibody therapy must be administered within 7 days of symptom onset. Virus Antibody Spike protein Antibodies block the virus from entering the cell or enable it to be destroyed.

What is Ly6C a marker for? ›

Ly6c is an antigen commonly used to differentiate between classical and non-classical monocytes/macrophages. Here we show its potential as a marker of the mouse vasculature, particularly of the retinal vascular plexuses.

What do Ly6C high monocytes do? ›

Monocytes expressing high levels of Ly6C (Ly6Chi monocytes) have proinflammatory and antimicrobial functions and express high levels of C-C che- mokine receptor 2 (CCR2) and low levels of CX3C chemo- kine receptor 1 (CX3CR1; CCR2hiCX3CR1low).

What is the best marker for neutrophils? ›

Cell Markers

Neutrophils can be distinguished from other granulocytes based on the expression of both CD15 and CD16/FcγRIII and a lack of CD14. In addition, CD66b/CEACAM-8, CD11b/Integrin alpha M, CD33, and myeloperoxidase are other markers that can be used to identify neutrophils.

What cancers can monoclonal antibodies treat? ›

Examples of MABS that work in this way include:
  • rituximab (Mabthera) – a treatment for chronic lymphocytic leukaemia (CLL) and some types of non Hodgkin lymphoma.
  • cetuximab (Erbitux) – a treatment for advanced bowel cancer and head and neck cancer.
  • trastuzumab (Herceptin) – used to treat breast cancer and stomach cancer.

Where do monoclonal antibodies come from? ›

Monoclonal antibodies (mAbs) can be made in animals immunized with purified protein, peptides, or bacterial or mammalian fusion proteins. The majority of monoclonal antibodies are made using fusion proteins because they can be produced in large quantities and most closely resemble, or are identical to, native protein.

What is the blood test for monoclonal antibodies? ›

Serum protein electrophoresis (SPEP) is a test that measures the antibodies in the blood and can find a monoclonal antibody. Another test, called immunofixation or immunoelectrophoresis, is used to determine the exact type of abnormal antibody (IgG. IgA or some other type).

What patients should get monoclonal antibodies? ›

The FDA authorized the use of these monoclonal antibody therapies to treat mild-to-moderate COVID-19 in adults and pediatric patients when both of these apply: They have a positive COVID-19 test result. They're at high risk for progressing to severe COVID-19, hospitalization, or both.

What are the diagnostic applications of monoclonal antibodies? ›

Monoclonal antibodies have two main applications - diagnostic and therapeutic. For diagnosis, they are used in assays and imaging to detect hormones, tumors, infections, and other biomarkers.

What is monoclonal disease? ›

Monoclonal gammopathies are conditions in which abnormal proteins (antibodies) are found in the blood. These proteins are made from a small number of plasma cells in the bone marrow.

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